Cytochalasin B-induced triploidy in mouse oocytes fertilized in vitro

Induction of triploidy in the mouse by cytochalasin B.

Mouse eggs fertilized in vivo were treated with cytochalasin B in vitro (5 mug/ml of culture medium) at the moment of extrusion of the second polar body (2-5, 3-0, 3-5 h after copulation). Cytochalasin B inhibits cytokinesis of the second maturation division, so that triploid digynic eggs are formed in over 50% of treated eggs. Triploid eggs were transplanted to the oviducts of recipients. On t...

P-229: Chromosomal Analysis of Parthenogenetic Mouse Embryos Generated from In Vitro Activated Oocytes by Hydrostatic Pressure and Ethanol and Cytochalasin B

Background: Studies of preimplantation stage embryos by classic cytogenetic techniques have limitations, starting with the need for good metaphase stage when only one third of all analyzed embryos may show good quality metaphases. The incidence of chromosome anomalies in embryos produced by in vitro procedures is generally higher than that of embryos formed in vivo. Pressure specifically affect...

chromosomal abnormalities in failed fertilized oocytes following in vitro fertilization

introduction: the types and incidence of the chromosomal abnormalities in failed fertilized oocytes that were taken after routine in vitro fertilization (ivf) and intracytoplasmic sperm injection (icsi) programs were investingated in patients referred the royan institute. materials and method: in this study, 364 air-dried preparation of failed fertilized oocytes after either ivf (91 samples) or...

P-87: High Fat Diet Affects In Vitro Maturation and In Vitro Fertilization of Mouse Oocytes

Background: Physiological effects of obesity are increasingly common in women of reproductive age and are associated with infertility. Obese women have dysfunction in hypothalamic-pituitary-ovarian axis, menstrual cycle and are up to three times more likely to have decreased oligo-/anovulation. The purpose of present study was to evaluate the high fat diet on IVM and IVF outcomes in mouse. Mate...

Reproductive efficiency of human oocytes fertilized in vitro